Hole array layer structure, pre-coating method, film-forming method, and related devices and processes

Sep 16, 2022

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1. The present technology belongs to the technical field of biological detection, and in particular relates to a hole array layer structure, a precoating method, a film forming method and a related device.

Background technique:

2. In nanopore sequencing, analytes such as biological macromolecules are driven through a nanopore sequencing channel embedded in a supporting membrane (such as a lipid membrane) by applying a certain potential, and different chemical groups are used to sequence through the nanopore. The channel induces a current change that identifies biomacromolecules passing through the nanopore sequencing channel. The structure of forming nanopores is usually divided into upper and lower layers, and the upper layers are connected to each other. However, after the film formation process, the interconnected structure of the upper layer can easily cause the medium to flow in each nanopore, which seriously affects the stability of the film after formation. .

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